Journal: Scientific Reports
Article Title: Illuminating dynamic neutrophil trans-epithelial migration with micro-optical coherence tomography
doi: 10.1038/srep45789
Figure Lengend Snippet: ( A ) and ( B ) Initial and final 3D renderings of 1 mm region during migration (room temperature). Neutrophils (red arrows) added to the basolateral side of the Transwell (yellow arrows) and epithelial barrier (green arrows) at time 0 penetrate the epithelium in discrete clusters (blue arrows) over a 2 hour imaging period. A fraction of the neutrophils detach from the clusters and fall to the glass surface (violet arrows). See for a complete time lapse at 10-minute intervals. ( C – F ) Comparison of quantitative migration results obtained by μOCT image analysis and myeloperixodase (MPO) assay; migration across T84 monolayers in response to fMLP. μOCT results include negative control (HBSS only). Error bars in all cases represent SEM, and PMN totals were scaled to represent the area of an entire Transwell filter. ( C ) and ( D ) Number of PMNs attached to epithelium as measured by μOCT and MPO respectively. ( E ) and F) Number of PMNs detached from epithelium as measured by μOCT and MPO respectively.
Article Snippet: Colorectal carcinoma human intestinal epithelial cell line T84 was purchased from American Tissue Culture Collection (ATCC, Manassas, VA).
Techniques: Migration, Imaging, Comparison, MPO Assay, Negative Control